pAc5.1A-Dm-mir-12
(Plasmid
#22760)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22760 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAc5.1A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5348
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Vector typeDrosophila expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDh5 alpha and XL1-blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemicro RNA 12
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Alt namemiR-12
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SpeciesD. melanogaster (fly); primary transcript
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Insert Size (bp)474
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GenBank IDNR_002128
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Entrez Genemir-12 (a.k.a. Dmel_CR33076, CR33076, Dmel\CR33076, MiR-12, Mir-12, miR-12, miR12, pri-miR-12)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Ac5 forward primer site
- 3′ sequencing primer BGH reverse priming site (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1A-Dm-mir-12 was a gift from Elisa Izaurralde (Addgene plasmid # 22760 ; http://n2t.net/addgene:22760 ; RRID:Addgene_22760) -
For your References section:
A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E. RNA. 2005 Nov . 11(11):1640-7. 10.1261/rna.2191905 PubMed 16177138
Map uploaded by the depositor.
