pAC025
(Plasmid
#227597)
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Purpose(Empty Backbone) Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAC025
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin ; BFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC025 was a gift from Brittany Adamson (Addgene plasmid # 227597 ; http://n2t.net/addgene:227597 ; RRID:Addgene_227597) -
For your References section:
A benchmarked, high-efficiency prime editing platform for multiplexed dropout screening. Cirincione A, Simpson D, Yan W, McNulty R, Ravisankar P, Solley SC, Yan J, Lim F, Farley EK, Singh M, Adamson B. Nat Methods. 2024 Nov 19. doi: 10.1038/s41592-024-02502-4. 10.1038/s41592-024-02502-4 PubMed 39562753
Map uploaded by the depositor.
