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Addgene

Lifeact-3xmNG::HIS3
(Plasmid #227596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS303
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lifeact-3xmNeonGreen::HIS3
  • Alt name
    ABP140
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Truncated ABP140 (aa 1-17 only)
  • Promoter Abp140
  • Tag / Fusion Protein
    • 3xmNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lifeact-3xmNG::HIS3 was a gift from Bruce Goode (Addgene plasmid # 227596 ; http://n2t.net/addgene:227596 ; RRID:Addgene_227596)

  • For your References section:

    Improved tools for live imaging of F-actin structures in yeast. Wirshing ACE, Goode BL. Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi: 10.1091/mbc.E24-05-0212-T. Epub 2024 Jul 18. 10.1091/mbc.E24-05-0212-T PubMed 39024291
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