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Addgene

pHelp
(Plasmid #227583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227583 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    guide RNA scaffold

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelp was a gift from Chengzhang Fu (Addgene plasmid # 227583 ; http://n2t.net/addgene:227583 ; RRID:Addgene_227583)

  • For your References section:

    Autologous DNA mobilization and multiplication expedite natural products discovery from bacteria. Xie F, Zhao H, Liu J, Yang X, Neuber M, Agrawal AA, Kaur A, Herrmann J, Kalinina OV, Wei X, Muller R, Fu C. Science. 2024 Dec 13;386(6727):eabq7333. doi: 10.1126/science.abq7333. Epub 2024 Dec 13. 10.1126/science.abq7333 PubMed 39666857
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