pRel
(Plasmid
#227581)
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PurposeRelease plasmid with Cas9 working cassette, apramycin resistance, and pSG5 replicon
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGM1190
- Total vector size (bp) 10970
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRel was a gift from Chengzhang Fu (Addgene plasmid # 227581 ; http://n2t.net/addgene:227581 ; RRID:Addgene_227581) -
For your References section:
Autologous DNA mobilization and multiplication expedite natural products discovery from bacteria. Xie F, Zhao H, Liu J, Yang X, Neuber M, Agrawal AA, Kaur A, Herrmann J, Kalinina OV, Wei X, Muller R, Fu C. Science. 2024 Dec 13;386(6727):eabq7333. doi: 10.1126/science.abq7333. Epub 2024 Dec 13. 10.1126/science.abq7333 PubMed 39666857
Map uploaded by the depositor.
