Orai1-myc E106D MO70
(Plasmid
#22755)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMO70
- Backbone size w/o insert (bp) 7400
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOrai1 E106D
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Alt nameOrai1
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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MutationE106D
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Entrez GeneORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lab Plasmid #SF102
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Orai1-myc E106D MO70 was a gift from Anjana Rao (Addgene plasmid # 22755 ; http://n2t.net/addgene:22755 ; RRID:Addgene_22755) -
For your References section:
Orai1 is an essential pore subunit of the CRAC channel. Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG. Nature. 2006 Sep 14. 443(7108):230-3. 10.1038/nature05122 PubMed 16921383
