Orai1-myc E106Q MO70
(Plasmid #22754)

• Depositing Lab: Anjana Rao
• Publication: Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Backbone

• Vector backbone: MO70
• Backbone size w/o insert (bp): 7400
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Orai1 E106Q
• Alt name: Orai1
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: E106Q
• Entrez Gene: ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
• Tag / Fusion Protein:
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: IRES-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lab Plasmid #SF101