Skip to main content
Addgene

myc-Orai1 E190A pHAGE
(Plasmid #22753)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Gift of Richard Mulligan
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Orai1 E190A
  • Alt name
    Orai1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    E190A
  • Entrez Gene
    ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For lentiviral transduction, N-terminally myc-tagged versions of Orai1 were directly cloned NotI-BamHI into the bicistronic pHAGE-UBC-eGFP-IRES-eGFP vector (R. Mulligan, Harvard Medical School), replacing the first eGFP with myc-Orai1.

Lab Plasmid # SF214. The Author's sequence includes the ORF plus 60 nt of vector boundary 3' and 5'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myc-Orai1 E190A pHAGE was a gift from Anjana Rao (Addgene plasmid # 22753 ; http://n2t.net/addgene:22753 ; RRID:Addgene_22753)

  • For your References section:

    Orai1 is an essential pore subunit of the CRAC channel. Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG. Nature. 2006 Sep 14. 443(7108):230-3. 10.1038/nature05122 PubMed 16921383
Related items: