AAV-FLEX-CAG_G-PTEN
(Plasmid #227438)

• Purpose: An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.
• Depositing Lab: Tal Laviv
• Publication: Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi: 10.1038/s41592-025-02610-9. Epub 2025 Feb 20.

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Backbone

• Vector backbone: pAAV FLEX CAG
• Backbone size w/o insert (bp): 4400
• Total vector size (bp): 2600
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G-PTEN sensor
• Alt name: mEGFP-PTEN-sREACh
• Species: R. norvegicus (rat)
• Mutation: R14G
• Entrez Gene: Pten (a.k.a. MMAC1, Mmac, TEP1)
• Promoter: pCAG
• Tags / Fusion Proteins:
  • mEGFP (N terminal on insert)
  • CD-sREACh (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CAG For

Resource Information

• Supplemental Documents:
  • AAV.FLEX.pCAG-G-PTEN_corected.gbk
• A portion of this plasmid was derived from a plasmid made by: The PTEN gene was obtained from Addgene plasmid #110181 pEGFP-PTEN

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression is Cre dependent.

Please visit https://doi.org/10.1101/2024.10.04.616644 for bioRxiv preprint.