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Addgene

CAG_G-PTEN
(Plasmid #227436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CAG CyRFP1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G-PTEN sensor, mEGFP-PTEN-sREACh
  • Species
    R. norvegicus (rat)
  • Mutation
    R14G
  • Entrez Gene
    Pten (a.k.a. MMAC1, Mmac, TEP1)
  • Promoter CAG
  • Tags / Fusion Proteins
    • mEGFP, (N terminal on insert)
    • sREACh (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The PTEN gene was obtained from Addgene plasmid #110181 pEGFP-PTEN

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.04.616644 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG_G-PTEN was a gift from Tal Laviv (Addgene plasmid # 227436 ; http://n2t.net/addgene:227436 ; RRID:Addgene_227436)

  • For your References section:

    Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain. Kagan T, Gabay M, Meenakshisundaram A, Levi Y, Eid S, Malchenko N, Maman M, Nitzan A, Ravotto L, Zaidel-Bar R, Eickholt BJ, Gal M, Laviv T. Nat Methods. 2025 Apr;22(4):764-777. doi: 10.1038/s41592-025-02610-9. Epub 2025 Feb 20. 10.1038/s41592-025-02610-9 PubMed 39979596
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