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CMV_R-PTEN_4A
(Plasmid #227435)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227435 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFP C1
  • Total vector size (bp) -1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    R-PTEN 4A
  • Alt name
    mCyRFP2-PTEN-mMaroon with 4A mutation
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Pten (a.k.a. MMAC1, Mmac, TEP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCyRFP2, mMaroon

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.04.616644 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV_R-PTEN_4A was a gift from Tal Laviv (Addgene plasmid # 227435 ; http://n2t.net/addgene:227435 ; RRID:Addgene_227435)

  • For your References section:

    Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain. Kagan T, Gabay M, Meenakshisundaram A, Levi Y, Eid S, Malchenko N, Maman M, Nitzan A, Ravotto L, Zaidel-Bar R, Eickholt BJ, Gal M, Laviv T. Nat Methods. 2025 Apr;22(4):764-777. doi: 10.1038/s41592-025-02610-9. Epub 2025 Feb 20. 10.1038/s41592-025-02610-9 PubMed 39979596
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