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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV-DsRed-Express
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha. Normal bacterial growing conditions
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemodified pCMV-DsRed-Express (Clontech)
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Insert Size (bp)4633
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned and modified in Hudson lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
modified pCMV-DsRed-Express (Clontech)) Have inserted 2 sites (NheI and BamHI upstream of DsRed-Express cassette and 2 sites downstream (BglII and NheI) = NheI-BamHI-DsRed-Express-BglII-EcoRI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDsRed-Sensor was a gift from Lynn Hudson (Addgene plasmid # 22743 ; http://n2t.net/addgene:22743 ; RRID:Addgene_22743) -
For your References section:
Identification of dynamically regulated microRNA and mRNA networks in developing oligodendrocytes. Lau P, Verrier JD, Nielsen JA, Johnson KR, Notterpek L, Hudson LD. J Neurosci. 2008 Nov 5. 28(45):11720-30. 10.1523/JNEUROSCI.1932-08.2008 PubMed 18987208