AAV-NG-CBE C
(Plasmid #227422)

• Purpose: expresses the C-terminal of AAV-NG-CBE
• Depositing Lab: Yang Sun
• Publication: Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. doi: 10.1002/advs.202405628.

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Backbone

• Vector backbone: px601
• Total vector size (bp): 7654
• Modifications to backbone: Backbone sourced from Addgene plasmid #137176
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAV-NG-CBE C-terminal
• Species: Synthetic
• Promoter: Cbh

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gcaagaggtaagggtttaaggg
• 3′ sequencing primer: U6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David Liu, backbone sourced from Addgene plasmid #137176.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry