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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22741 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA-6.2 GW/EmGFP miR-neg control
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5699
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemicroRNA 9
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Alt namemir9
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Insert Size (bp)65
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDNA cloned within Hudson lab
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA6.2-EmGFP-mir9 was a gift from Lynn Hudson (Addgene plasmid # 22741 ; http://n2t.net/addgene:22741 ; RRID:Addgene_22741) -
For your References section:
Identification of dynamically regulated microRNA and mRNA networks in developing oligodendrocytes. Lau P, Verrier JD, Nielsen JA, Johnson KR, Notterpek L, Hudson LD. J Neurosci. 2008 Nov 5. 28(45):11720-30. 10.1523/JNEUROSCI.1932-08.2008 PubMed 18987208