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Addgene

pCR-Myt1NB580
(Plasmid #22740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II TOPO
  • Backbone size w/o insert (bp) 3500
  • Vector type
    PCR cloning vector
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha. Standard growing conditons
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myelin Transcription Factor 1
  • Alt name
    Myt1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    580
  • Entrez Gene
    Myt1 (a.k.a. Nzf2, Nzf2a, Nzf2b, Nztf2)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO (not destroyed)
  • 3′ cloning site TOPO (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA cloned within Hudson lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

580bp Myt1 insert can be released with EcoRI. This is the 580bp cDNA probe for Northern blots. Paper is in preparation. Tentatively titled "The Myelin Transcription Factor 1 (Myt1) is Essential for Neural Development"

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-Myt1NB580 was a gift from Lynn Hudson (Addgene plasmid # 22740 ; http://n2t.net/addgene:22740 ; RRID:Addgene_22740)
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