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Addgene

pLenti CMV SNAP MDC1
(Plasmid #227375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV/TO GFP-MDC1
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MDC1, mediator of DNA damage checkpoint 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    MDC1 (a.k.a. NFBD1)
  • Tag / Fusion Protein
    • SNAP (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Eric Campeau (Addgene plasmid #26285).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector was generated by cloning (Genewiz) a synthesized SNAPf fragment (sequence from pBS-TRE-SNAPf-WPRE; Addgene plasmid #104106) with AgeI and BstBI restriction sites into the pLenti CMV/TO GFP-MDC1 (779-2) (Addgene plasmid #26285, gift from Eric Campeau) backbone, substituting SNAPf with eGFP at the N terminus of MDC1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV SNAP MDC1 was a gift from David Pellman (Addgene plasmid # 227375 ; http://n2t.net/addgene:227375 ; RRID:Addgene_227375)

  • For your References section:

    Heritable transcriptional defects from aberrations of nuclear architecture. Papathanasiou S, Mynhier NA, Liu S, Brunette G, Stokasimov E, Jacob E, Li L, Comenho C, van Steensel B, Buenrostro JD, Zhang CZ, Pellman D. Nature. 2023 Jul;619(7968):184-192. doi: 10.1038/s41586-023-06157-7. Epub 2023 Jun 7. 10.1038/s41586-023-06157-7 PubMed 37286600
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