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CuO-LacI-NLS–SNAPf
(Plasmid #227368)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227368 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-EF1-CymR-T2A-Puro
  • Backbone manufacturer
    System Biosciences
  • Total vector size (bp) 10851
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LacI NLS SNAPf
  • Species
    H. sapiens (human)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Takeshi Imai, Mirek Dundr, and System Biosciencces. CuO-LacI-NLS–SNAPf contains the coding sequence for the SNAPf-Tag (sequence from pBS-TRE-SNAPf-WPRE; Addgene plasmid #104106) followed in-frame with the coding sequence for LacI-NLS (sequence taken from Cherry-LacRep; Addgene plasmid #18985). This sequence was subcloned into pCDH-EF1-CymR-T2A-Puro (QM200VA-1, System Biosciences SBI) using NheI and BstBI restriction sites.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CuO-LacI-NLS–SNAPf was a gift from David Pellman (Addgene plasmid # 227368 ; http://n2t.net/addgene:227368 ; RRID:Addgene_227368)

  • For your References section:

    Heritable transcriptional defects from aberrations of nuclear architecture. Papathanasiou S, Mynhier NA, Liu S, Brunette G, Stokasimov E, Jacob E, Li L, Comenho C, van Steensel B, Buenrostro JD, Zhang CZ, Pellman D. Nature. 2023 Jul;619(7968):184-192. doi: 10.1038/s41586-023-06157-7. Epub 2023 Jun 7. 10.1038/s41586-023-06157-7 PubMed 37286600
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