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pGEX-parkin V224A
(Plasmid #227353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Amersham
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Parkin V224A
  • Alt name
    PARK2
  • Species
    H. sapiens (human)
  • Mutation
    Codon usage optimized for E.coli expression. Changed valine 224 to alanine.
  • Entrez Gene
    PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
  • Tag / Fusion Protein
    • GST (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: BamHI (unknown if destroyed), 3' Cloning Site: XhoI (unknown if destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-parkin V224A was a gift from Kalle Gehring (Addgene plasmid # 227353 ; http://n2t.net/addgene:227353 ; RRID:Addgene_227353)

  • For your References section:

    Naturally occurring hyperactive variants of human parkin. Huq TS, Luo J, Fakih R, Sauve V, Gehring K. Commun Biol. 2024 Aug 8;7(1):961. doi: 10.1038/s42003-024-06656-x. 10.1038/s42003-024-06656-x PubMed 39117722
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