peGFP-N2-rPrmp
(Plasmid #22735)

• Depositing Lab: Lynn Hudson
• Publication: Nielsen et al J Neurosci. 2006 Sep 27. 26(39):9881-91.

Backbone

• Vector backbone: peGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Proline-rich transmembrane protein
• Alt name: Prmp
• Species: R. norvegicus (rat)
• Insert Size (bp): 618
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primer (#6479-1): 749–728
• 3′ sequencing primer: EGFP-C Sequencing Primer (#6478-1): 1336–1357

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This DNA was cloned in Hudson lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry