pMDW139
(Plasmid #227336)

• Purpose: Template for in vitro Pol II transcription
• Depositing Lab: Michelle Wang
• Publication: Template for in vitro Pol II transcription (unpublished)

Backbone

• Vector backbone: pUC119
• Backbone size w/o insert (bp): 6667
• Total vector size (bp): 7339
• Modifications to backbone: pUC119 vector with A1 promoter of phage T7 preceding a portion of the E. coli trp leader and the rrnB T1 terminator
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: T7 A1 promoter interferes with replication. For best culture growth, do not go right from glycerol, streak out and pick new colonies or re-transform.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gap DNA
• Species: Bacterial
• Insert Size (bp): 672
• Promoter: Plac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: DraIII-HF (unknown if destroyed)
• 3′ cloning site: BstXI (unknown if destroyed)
• 5′ sequencing primer: 5’-ACT ACA CCT AGT GCA GGG CGC GTA CTA-3’
• 3′ sequencing primer: 5’-TAA TCC AAA TCG ATG GCC CGA AGA GTG-3’

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R Landick, modified pUC119 vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry