pLCA.66/2272
(Plasmid #22733)

• Depositing Lab: Mark Magnuson
• Publication: Chen et al Dis Model Mech. 2011 Feb 14. ():.

Backbone

• Vector backbone: pBluescript KS+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2969
• Vector type: Cre/Lox ; BAC recombineering
• Selectable markers: Neomycin (select with G418) ; ganciclovir

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 1. EL350 – for recombineering transformation steps 2. DH5alfa, XL1-blue, or other competent bacterial cell line – for non-recombineering transformation steps
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lox66-pU(delta)TK-EM7Neo-Lox2272
• Insert Size (bp): 3697
• Mutation: This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid names and lox sites have been corrected since publication.