pMCS.71/2272
(Plasmid #22732)

• Depositing Lab: Mark Magnuson
• Publication: Chen et al Dis Model Mech. 2011 Feb 14. ():.

Backbone

• Vector backbone: pBluescript KS+
• Backbone size w/o insert (bp): 2905
• Vector type: Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alfa, XL1-blue, or other competent bacterial cell line
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lox71-MCS-Lox2272
• Insert Size (bp): 150
• Mutation: This is a basal exchange vector for RMCE containing heterotypic LoxP sites. A lox71 site, flanked by Sac I and Not I, and a lox2272 site, flanked by Xho I and Kpn I, were inserted into the same sites of pBluescript KS+.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sac1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid names and lox sites have been corrected since publication.