qTAG-C-mStayGold-EFS-Puro-PLK4
(Plasmid
#227311)
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PurposeDonor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 227311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2686
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Vector typeMammalian Expression, CRISPR ; Donor Template
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette
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Alt namePLK4
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SpeciesH. sapiens (human)
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Insert Size (bp)3107
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Entrez GenePLK4 (a.k.a. MCCRP2, SAK, STK18)
- Promoter Promoterless
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CTGCAAGGCGATTAAGTTGGGTAAC
- 3′ sequencing primer GGCTCGTATGTTGTGTGGAATTGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310): https://www.addgene.org/227310/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
qTAG-C-mStayGold-EFS-Puro-PLK4 was a gift from Laurence Pelletier (Addgene plasmid # 227311 ; http://n2t.net/addgene:227311 ; RRID:Addgene_227311) -
For your References section:
qTAG: An adaptable CRISPR-based endogenous tagging protocol using optimized repair cassettes. Philip R, Sharma A, Matellan L, Erpf AC, Hsu W, Tkach JM, Wyatt HDM, Pelletier L. bioRxiv 10.1101/2023.11.01.565029