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Addgene

pX330-PITCh-VIM
(Plasmid #227301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227301 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330-BbsI-PITCh
  • Backbone manufacturer
    Peter Kaiser, Addgene #127875
  • Backbone size w/o insert (bp) 8947
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sgRNA Targeting C-terminus of VIM
  • Alt name
    VIM
  • gRNA/shRNA sequence
    GCGCAAGATAGATTTGGAAT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Entrez Gene
    VIM
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330-PITCh-VIM was a gift from Laurence Pelletier (Addgene plasmid # 227301 ; http://n2t.net/addgene:227301 ; RRID:Addgene_227301)

  • For your References section:

    qTAG: An adaptable CRISPR-based endogenous tagging protocol using optimized repair cassettes. Philip R, Sharma A, Matellan L, Erpf AC, Hsu W, Tkach JM, Wyatt HDM, Pelletier L. bioRxiv 10.1101/2023.11.01.565029
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