pMCS.DT-A
(Plasmid #22730)

• Depositing Lab: Mark Magnuson
• Publication: Chen et al Dis Model Mech. 2011 Feb 14. ():.

Backbone

• Vector backbone: pBluescript KS+
• Backbone size w/o insert (bp): 2995
• Vector type: BAC recombineering
• Selectable markers: diphtheria toxin A

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 1. EL350 – for recombineering transformation steps 2. DH5alfa, XL1-blue, or other competent bacterial cell line – for non-recombineering transformation steps
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DT-A
• Insert Size (bp): 1069
• Mutation: This is a gap-repair vector designed for retrieving the targeted region of BAC clones to construct gene targeting vectors using a recombineering assembly strategy. The plasmid contains a diphtheria toxin selection gene, DT-A, cloned between the Kpn I and Sal I restriction enzyme sites of pBluescript KS+.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: Sal1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry