Skip to main content
Addgene

qTAG-C-Solo-mStayGold-CANX
(Plasmid #227280)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Mammalian Expression, CRISPR ; Donor Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CANX Homology Arms flanking a mStayGold Tag
  • Alt name
    CANX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1847
  • Entrez Gene
    CANX (a.k.a. CNX, IP90, P90)
  • Promoter Promoterless

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTGCAAGGCGATTAAGTTGGGTAAC
  • 3′ sequencing primer GGCTCGTATGTTGTGTGGAATTGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279): https://www.addgene.org/227279/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    qTAG-C-Solo-mStayGold-CANX was a gift from Laurence Pelletier (Addgene plasmid # 227280 ; http://n2t.net/addgene:227280 ; RRID:Addgene_227280)

  • For your References section:

    qTAG: An adaptable CRISPR-based endogenous tagging protocol using optimized repair cassettes. Philip R, Sharma A, Matellan L, Erpf AC, Hsu W, Tkach JM, Wyatt HDM, Pelletier L. bioRxiv 10.1101/2023.11.01.565029
Related items: