pMXs-p53P275S
(Plasmid #22726)

• Depositing Lab: Shinya Yamanaka
• Publication: Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Backbone

• Vector backbone: pMXs-gw
• Backbone size w/o insert (bp): 4600
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trp53
• Alt name: p53
• Species: M. musculus (mouse)
• Insert Size (bp): 1252
• Mutation: P275S
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB1 (unknown if destroyed)
• 3′ cloning site: attB2 (unknown if destroyed)
• 5′ sequencing primer: pMX-S1811
• 3′ sequencing primer: pMXs-AS3200

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

additional article reference: de Vries, A. et al. Targeted point mutations of p53 lead to dominant-negative inhibition of wild-type p53 function. Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2948-53. Epub 2002 Feb 26.