pEC1.2/EC1.1_Cas9i_TagRFP
(Plasmid
#227249)
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Purpose(Empty Backbone) CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHEE401E
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Backbone manufacturerQi-Jun Chen, Addgene #71287
- Backbone size (bp) 10000
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Modifications to backboneReplacement of spCas9 with Cas9i and Hygromycin selection marker with TagRFP seed selection marker near the RB of the T-DNA
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Vector typePlant Expression, CRISPR
- Promoter pEC1.2/EC1.1
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Selectable markersTagRFP seed selection
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySylvestre Marillonnet
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEC1.2/EC1.1_Cas9i_TagRFP was a gift from Christian Fankhauser (Addgene plasmid # 227249 ; http://n2t.net/addgene:227249 ; RRID:Addgene_227249)
Map uploaded by the depositor.
