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Addgene

pMDW133
(Plasmid #227248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 2686
  • Modifications to backbone
    12bp removed by enzyme digestion
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lambda DNA
  • Species
    Escherichia coli bacteriophage (Bacteriophage lambda cI857 Sam7
  • Insert Size (bp)
    11552
  • Promoter Plac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI-HF (unknown if destroyed)
  • 3′ cloning site KpnI-HF (unknown if destroyed)
  • 5′ sequencing primer no primers used
  • 3′ sequencing primer no primers used
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    New England Biolabs (NEB-Item#N3011S). The phage is isolated from the heat-inducible lysogen E.coli l cI857 S7. The DNA is isolated from the purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDW133 was a gift from Michelle Wang (Addgene plasmid # 227248 ; http://n2t.net/addgene:227248 ; RRID:Addgene_227248)
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