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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]
(Plasmid #227245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2000
  • Total vector size (bp) 7750
  • Vector type
    Mammalian Expression, Cre/Lox, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Tet-On 3G transactivator protein
  • Alt name
    TetOn3G
  • Species
    Synthetic
  • Insert Size (bp)
    747
  • Promoter EF1α

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Enhanced green fluorescent protein
  • Alt name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter pTRE3G

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35] was a gift from Jae Seong Lee (Addgene plasmid # 227245 ; http://n2t.net/addgene:227245 ; RRID:Addgene_227245)

  • For your References section:

    A precise and sustainable doxycycline-inducible cell line development platform for reliable mammalian cell engineering with gain-of-function mutations. Shin SW, Min H, Kim J, Lee JS. Metab Eng. 2024 Sep 5;86:12-28. doi: 10.1016/j.ymben.2024.09.004. 10.1016/j.ymben.2024.09.004 PubMed 39242074
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