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Addgene

WT USP7 UBL-12
(Plasmid #227230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b (thrombin site was replaced with TEV-site)
  • Backbone size w/o insert (bp) 5714
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7)
  • Alt name
    HAUSP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    726
  • Mutation
    aa 535-775
  • Entrez Gene
    USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
  • Promoter T7-promoter
  • Tag / Fusion Protein
    • 6His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UBL-12 domain of USP7 WT

Plasmid is also associated with the following publications:
https://doi.org/10.1016/j.jmb.2020.166733
https://doi.org/10.1074/jbc.m115.664805

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WT USP7 UBL-12 was a gift from Irina Bezsonova (Addgene plasmid # 227230 ; http://n2t.net/addgene:227230 ; RRID:Addgene_227230)

  • For your References section:

    Nuclear magnetic resonance spectral data of the USP7 TRAF and UBL1-2 domains in complex with DNA polymerase iota peptides. Valles GJ, Ashton NW, Jaiswal N, Woodgate R, Bezsonova I. Data Brief. 2020 Dec 23;34:106680. doi: 10.1016/j.dib.2020.106680. eCollection 2021 Feb. 10.1016/j.dib.2020.106680 PubMed 33437851
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