R152C USP7 TRAF
(Plasmid
#227229)
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PurposeExpresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b
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Backbone manufacturerNovagen, 69865-6
- Backbone size w/o insert (bp) 5368
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUSP7 (Ubiquitin carboxyl-terminal hydrolase 7)
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Alt nameHAUSP
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SpeciesH. sapiens (human)
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Insert Size (bp)432
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MutationR152C; aa 62-205
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Entrez GeneUSP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
- Promoter T7-promoter
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Tag
/ Fusion Protein
- 6His (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TRAF-like domain of USP7 Hao-Fountain patient mutation R152C
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R152C USP7 TRAF was a gift from Irina Bezsonova (Addgene plasmid # 227229 ; http://n2t.net/addgene:227229 ; RRID:Addgene_227229)
