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Addgene

K420E USP7 catalytic
(Plasmid #227210)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227210 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a-LIC Vector (GenBank accession EF442785)
  • Backbone manufacturer
    Structural Genomics Consortium, Toronto
  • Backbone size w/o insert (bp) 5306
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7)
  • Alt name
    HAUSP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1062
  • Mutation
    K420E; aa 207-560
  • Entrez Gene
    USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
  • Promoter T7-promoter
  • Tag / Fusion Protein
    • 6His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

USP7 Catalytic domain Hao-Fountain patient mutation K420E

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K420E USP7 catalytic was a gift from Irina Bezsonova (Addgene plasmid # 227210 ; http://n2t.net/addgene:227210 ; RRID:Addgene_227210)
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