WT USP7 catalytic
(Plasmid #227201)

• Purpose: Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
• Depositing Lab: Irina Bezsonova
• Publication: Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.11.523550. doi: 10.1101/2023.01.11.523550.

Backbone

• Vector backbone: pET28a-LIC Vector (GenBank accession EF442785)
• Backbone manufacturer: Structural Genomics Consortium, Toronto
• Backbone size w/o insert (bp): 5306
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: USP7 (Ubiquitin carboxyl-terminal hydrolase 7)
• Alt name: HAUSP
• Species: H. sapiens (human)
• Insert Size (bp): 1062
• Mutation: aa 207-560
• Entrez Gene: USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
• Promoter: T7-promoter
• Tag / Fusion Protein:
  • 6His (N terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

USP7 Catalytic domain WT

Please visit https://doi.org/10.1101/2023.01.11.523550 for bioRxiv preprint.

Plasmid is also associated with the following publications:
https://doi.org/10.1371/journal.pone.0189744
https://doi.org/10.1016/j.chembiol.2017.09.004