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Addgene

WT USP7 catalytic
(Plasmid #227201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a-LIC Vector (GenBank accession EF442785)
  • Backbone manufacturer
    Structural Genomics Consortium, Toronto
  • Backbone size w/o insert (bp) 5306
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7)
  • Alt name
    HAUSP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1062
  • Mutation
    aa 207-560
  • Entrez Gene
    USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
  • Promoter T7-promoter
  • Tag / Fusion Protein
    • 6His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

USP7 Catalytic domain WT

Please visit https://doi.org/10.1101/2023.01.11.523550 for bioRxiv preprint.

Plasmid is also associated with the following publications:
https://doi.org/10.1371/journal.pone.0189744
https://doi.org/10.1016/j.chembiol.2017.09.004

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WT USP7 catalytic was a gift from Irina Bezsonova (Addgene plasmid # 227201 ; http://n2t.net/addgene:227201 ; RRID:Addgene_227201)

  • For your References section:

    Activation Dynamics of Ubiquitin Specific Protease 7. Valles GJ, Jaiswal N, Korzhnev DM, Bezsonova I. bioRxiv [Preprint]. 2023 Jan 12:2023.01.11.523550. doi: 10.1101/2023.01.11.523550. 10.1101/2023.01.11.523550 PubMed 36711877
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