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Addgene

pID3.01B
(Plasmid #22720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Backbone size w/o insert (bp) 16320
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1713

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (unknown if destroyed)
  • 3′ cloning site attR2 (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pID3.01B was a gift from Geraldine Seydoux (Addgene plasmid # 22720 ; http://n2t.net/addgene:22720 ; RRID:Addgene_22720)

  • For your References section:

    Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline. D'Agostino I, Merritt C, Chen PL, Seydoux G, Subramaniam K. Dev Biol. 2006 Apr 1. 292(1):244-52. 10.1016/j.ydbio.2005.11.046 PubMed 16499902
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