pMDW108
(Plasmid #227194)

• Purpose: Template for multi-nucleosome array
• Depositing Lab: Michelle Wang
• Publication: Le et al Cell. 2019 Oct 17;179(3):619-631.e15. doi: 10.1016/j.cell.2019.09.034.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 15366
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
• Alt name: p197NRL-64ex
• Species: Synthetic
• Insert Size (bp): 12680
• GenBank ID: n/a
• Promoter: Plac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bs1XI (unknown if destroyed)
• 3′ cloning site: BgII (unknown if destroyed)
• 5′ sequencing primer: no primers used
• 3′ sequencing primer: no primers used

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lowary and Widom.1998. Journal of Molecular Biology. (276)19-42.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry