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Addgene

pLIX401 APE1 CI
(Plasmid #227191)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227191 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLIX401
  • Backbone manufacturer
    Addgene
  • Total vector size (bp) 8653
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    apurinic/apyrimidinic endonuclease APE1
  • Alt name
    APEX1
  • Species
    H. sapiens (human)
  • Mutation
    H309N
  • Entrez Gene
    APEX1 (a.k.a. APE, APE1, APEN, APEX, APX, HAP1, REF1)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    cDNAs with APE1 was a gift from Dr. Wade Harper, Harvard University, Boston, MA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIX401 APE1 CI was a gift from David Pellman (Addgene plasmid # 227191 ; http://n2t.net/addgene:227191 ; RRID:Addgene_227191)
  • For your References section:

    Breakage of cytoplasmic chromosomes by pathological DNA base excision repair. Tang S, Stokasimov E, Cui Y, Pellman D. Nature. 2022 Jun;606(7916):930-936. doi: 10.1038/s41586-022-04767-1. Epub 2022 Apr 27. 10.1038/s41586-022-04767-1 PubMed 35477155