pLIX401 APE1 WT
(Plasmid
#227190)
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PurposeInducible lentiviral expression of WT APE1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 227190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLIX401
- Total vector size (bp) 8653
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameapurinic/apyrimidinic endonuclease APE1
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Alt nameAPEX1
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SpeciesH. sapiens (human)
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Entrez GeneAPEX1 (a.k.a. APE, APE1, APEN, APEX, APX, HAP1, REF1)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bycDNAs with APE1 was a gift from Dr. Wade Harper, Harvard University, Boston, MA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIX401 APE1 WT was a gift from David Pellman (Addgene plasmid # 227190 ; http://n2t.net/addgene:227190 ; RRID:Addgene_227190) -
For your References section:
Breakage of cytoplasmic chromosomes by pathological DNA base excision repair. Tang S, Stokasimov E, Cui Y, Pellman D. Nature. 2022 Jun;606(7916):930-936. doi: 10.1038/s41586-022-04767-1. Epub 2022 Apr 27. 10.1038/s41586-022-04767-1 PubMed 35477155