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Addgene

pKDC.118
(Plasmid #227188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LbuCas13a
  • Species
    L. buccalis

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKDC.118 was a gift from Seth Shipman (Addgene plasmid # 227188 ; http://n2t.net/addgene:227188 ; RRID:Addgene_227188)

  • For your References section:

    Continuous multiplexed phage genome editing using recombitrons. Fishman CB, Crawford KD, Bhattarai-Kline S, Poola D, Zhang K, Gonzalez-Delgado A, Rojas-Montero M, Shipman SL. Nat Biotechnol. 2024 Sep 5. doi: 10.1038/s41587-024-02370-5. 10.1038/s41587-024-02370-5 PubMed 39237706
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