Skip to main content
Addgene

pTRIP-UbC-Blast-2A-STING-mNeonGreen
(Plasmid #227185)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227185 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pTRIP
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STING1
  • Species
    H. sapiens (human)
  • Entrez Gene
    STING1 (a.k.a. ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING)
  • Promoter UbC
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gattcgtgaattgctgccct
  • 3′ sequencing primer atgctgattgtgcctggcta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a 313bp deletion at the 5' end of the UbC promoter. This deletion is not expected to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP-UbC-Blast-2A-STING-mNeonGreen was a gift from Nir Hacohen (Addgene plasmid # 227185 ; http://n2t.net/addgene:227185 ; RRID:Addgene_227185)

  • For your References section:

    ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling. Gentili M, Liu B, Papanastasiou M, Dele-Oni D, Schwartz MA, Carlson RJ, Al'Khafaji AM, Krug K, Brown A, Doench JG, Carr SA, Hacohen N. Nat Commun. 2023 Feb 4;14(1):611. doi: 10.1038/s41467-023-36132-9. 10.1038/s41467-023-36132-9 PubMed 36739287
Related items: