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Addgene

AAV-GFAP-TurboID
(Plasmid #227179)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV[Exp]
  • Backbone manufacturer
    Vector Builder
  • Total vector size (bp) 6296
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TurboID
  • Promoter GFAP_short
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • T2A-mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-GFAP-TurboID was a gift from Junmin Peng (Addgene plasmid # 227179 ; http://n2t.net/addgene:227179 ; RRID:Addgene_227179)

  • For your References section:

    Deep Single-Cell-Type Proteome Profiling of Mouse Brain by Nonsurgical AAV-Mediated Proximity Labeling. Sun X, Sun H, Han X, Chen PC, Jiao Y, Wu Z, Zhang X, Wang Z, Niu M, Yu K, Liu D, Dey KK, Mancieri A, Fu Y, Cho JH, Li Y, Poudel S, Branon TC, Ting AY, Peng J. Anal Chem. 2022 Apr 5;94(13):5325-5334. doi: 10.1021/acs.analchem.1c05212. Epub 2022 Mar 22. 10.1021/acs.analchem.1c05212 PubMed 35315655
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