AAV-CaMKII-TurboID
(Plasmid #227178)

• Purpose: Co-expression of FLAG-tagged TurboID and T2A-mCherry under the CaMKIIα short promoter by AAV vector
• Depositing Lab: Junmin Peng
• Publication: Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334. doi: 10.1021/acs.analchem.1c05212. Epub 2022 Mar 22.

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Backbone

• Vector backbone: pAAV[Exp]
• Backbone manufacturer: Vector Builder
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TurboID
• Promoter: CaMKIIa_short
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • T2A-mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry