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HA-EglN3 H196A-pcDNA3
(Plasmid #22717)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22717 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5445
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Egg laying Nine - 3
  • Alt name
    PHD3
  • Alt name
    prolyl Hydroxylase 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    774
  • Mutation
    mutant cDNA: point mutation at codon 196 was inserted to generate mutation H196A
  • GenBank ID
    BC010992
  • Entrez Gene
    EGLN3 (a.k.a. HIFP4H3, HIFPH3, PHD3)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

this mutant version of HA-EglN3 H196A-pcDNA3 can not be distinguished from its parental wt plasmid by restriction digest

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-EglN3 H196A-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 22717 ; http://n2t.net/addgene:22717 ; RRID:Addgene_22717)

  • For your References section:

    Neuronal apoptosis linked to EglN3 prolyl hydroxylase and familial pheochromocytoma genes: developmental culling and cancer. Lee S, Nakamura E, Yang H, Wei W, Linggi MS, Sajan MP, Farese RV, Freeman RS, Carter BD, Kaelin WG, Schlisio S. Cancer Cell. 2005 Aug . 8(2):155-67. 10.1016/j.ccr.2005.06.015 PubMed 16098468
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