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Addgene

pSuperGFP-cdc42-prenyl siRNA
(Plasmid #22715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuperGFP
  • Backbone manufacturer
    Oligoengine
  • Backbone size w/o insert (bp) 5429
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc42 siRNA
  • Alt name
    cdc42 prenyl siRNA
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    19
  • Entrez Gene
    Rac1
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

siRNA: GAAGAGCCGCAGGTGTGTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuperGFP-cdc42-prenyl siRNA was a gift from Alaa El-Husseini (Addgene plasmid # 22715 ; http://n2t.net/addgene:22715 ; RRID:Addgene_22715)
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