Skip to main content
Addgene

iNicSnFR12
(Plasmid #227142)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pHHM.X51
  • Backbone size w/o insert (bp) 2761
  • Total vector size (bp) 4444
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iNicSnFR12
  • Species
    Synthetic
  • Insert Size (bp)
    1683
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis-HA (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iNicSnFR12 was a gift from Henry Lester (Addgene plasmid # 227142 ; http://n2t.net/addgene:227142 ; RRID:Addgene_227142)

  • For your References section:

    Interactive computational and experimental approaches improve the sensitivity of periplasmic binding protein-based nicotine biosensors for measurements in biofluids. Haloi N, Huang S, Nichols AL, Fine EJ, Friesenhahn NJ, Marotta CB, Dougherty DA, Lindahl E, Howard RJ, Mayo SL, Lester HA. Protein Eng Des Sel. 2024 Jan 29;37:gzae003. doi: 10.1093/protein/gzae003. 10.1093/protein/gzae003 PubMed 38302088
Related items: