pmMyt1
(Plasmid #22713)

• Depositing Lab: Lynn Hudson
• Publication: Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-90.

Backbone

• Vector backbone: pCRII
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3948
• Vector type: cloning vector TT/AA

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Normal growth conditions
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Myelin Transcription Factor 1
• Alt name: Myt1
• Species: M. musculus (mouse)
• Insert Size (bp): 5466
• Entrez Gene: Myt1 (a.k.a. Nzf2, Nzf2a, Nzf2b, Nztf2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7 promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DNA cloned in Hudson laboratory
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The murine Myt1 cDNA (6 zinc fingers) had 3 base mutations within the coding region (bp 806, 837 and 4344). These sites were corrected using Stratagene QuickChang Site-Directed mutagenesis. The Myt1 was sequenced after the mutagenesis and found to be correct. There is still one mutation in the 5' UTR at bp 529 (START codon is bp 532)