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pAc5.1A-Dm-miR-1
(Plasmid #22711)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5348
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha and XL-1 blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    micro RNA 1
  • Alt name
    miR-1
  • Species
    D. melanogaster (fly); primary transcript
  • Insert Size (bp)
    484
  • GenBank ID
    NR_001675
  • Entrez Gene
    mir-1 (a.k.a. Dmel_CR32958, CR32958, Dmel\CR32958, DmiR-1, MiR-1, Mir-1, Mir1, dme-mir-1, dmiR-1, miR-1, miR1, mirR-1, transfrag 8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1A-Dm-miR-1 was a gift from Elisa Izaurralde (Addgene plasmid # 22711 ; http://n2t.net/addgene:22711 ; RRID:Addgene_22711)

  • For your References section:

    Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing. Eulalio A, Rehwinkel J, Stricker M, Huntzinger E, Yang SF, Doerks T, Dorner S, Bork P, Boutros M, Izaurralde E. Genes Dev. 2007 Oct 15. 21(20):2558-70. 10.1101/gad.443107 PubMed 17901217
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