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Addgene

TAK11
(Plasmid #227095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3 Basic
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 6355
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV Promoter and HIV-1 TAR region
  • Species
    HIV-1
  • Insert Size (bp)
    1499
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer OFM640
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAK11 was a gift from Farah Mustafa (Addgene plasmid # 227095 ; http://n2t.net/addgene:227095 ; RRID:Addgene_227095)

  • For your References section:

    Transactivation of the novel 5' cis-acting element of mouse mammary tumor virus (MMTV) by human retroviral transactivators Tat and Tax. Khader TA, Ahmad W, Akhlaq S, Panicker NG, Gull B, Baby J, Rizvi TA, Mustafa F. Commun Biol. 2024 Nov 16;7(1):1521. doi: 10.1038/s42003-024-07139-9. 10.1038/s42003-024-07139-9 PubMed 39550519
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