CPH3736_GST-TEV-Spt6p-tSH2-1223-1454
(Plasmid
#227088)
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PurposeBacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 227088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST15
- Backbone size w/o insert (bp) 5429
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namespt6-tsh2
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Alt nameYGR116W
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)688
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MutationR1282H, K1355A, K1435A
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Entrez GeneSPT6 (a.k.a. YGR116W, CRE2, SSN20)
- Promoter T7
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Tag
/ Fusion Protein
- GST-TEV_cleavage_site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CPH3736_GST-TEV-Spt6p-tSH2-1223-1454 was a gift from Chris Hill (Addgene plasmid # 227088 ; http://n2t.net/addgene:227088 ; RRID:Addgene_227088)