CPH3375_SF_Spt6p-tSH2-1223-1451_NLS
(Plasmid
#227084)
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PurposeGal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 227084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
- Backbone size w/o insert (bp) 6542
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namespt6-tsh2
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Alt nameYGR116W
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)723
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Entrez GeneSPT6 (a.k.a. YGR116W, CRE2, SSN20)
- Promoter Gal1-10
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Tags
/ Fusion Proteins
- STREPii-FLAG (N terminal on insert)
- SV40 NLS 1 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (unknown if destroyed)
- 3′ cloning site BamH1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CPH3375_SF_Spt6p-tSH2-1223-1451_NLS was a gift from Chris Hill (Addgene plasmid # 227084 ; http://n2t.net/addgene:227084 ; RRID:Addgene_227084)